Expression of interferon-inducible genes in RD-114 cells
نویسندگان
چکیده
منابع مشابه
Inducible Expression of Human Gamma Interferon
Background:The premature termination of high producer clones, which will be killed due to cell proliferation and proteins production antagonism, is one of the basic drawback in recombinant proteins technology. Furtheremore, it is supposed some toxic proteins like interferon which we intended to clone and express, inhibit host cells’ proliferation. So, it is necessary to tightly control IFN-γ pr...
متن کاملSuppressive effects of interferon on syncytium formation by RD-114 virus in human transformed cells.
The RD-114 virus rapidly induced syncytia in the human transformed cell lines, RSa, RSb and IFr. Treatment of the virus with heat or ultrasonic vibration completely eliminated the syncytium-forming activity. Irradiation with u.v.-light or treatment with beta-propiolactone (BPL) reduced but did not completely destroy the activity. Pre-treatment of the cells for 16 h with 25 to 500 units/ml of hu...
متن کاملinducible expression of human gamma interferon
background:the premature termination of high producer clones, which will be killed due to cell proliferation and proteins production antagonism, is one of the basic drawback in recombinant proteins technology. furtheremore, it is supposed some toxic proteins like interferon which we intended to clone and express, inhibit host cells’ proliferation. so, it is necessary to tightly control ifn-γ pr...
متن کاملFunctional correction of FA-C cells with FANCC suppresses the expression of interferon gamma-inducible genes.
Because hematopoietic cells derived from Fanconi anemia (FA) patients of the C-complementation group (FA-C) are hypersensitive to the inhibitory effects of interferon gamma (IFNgamma), the products of certain IFNgamma-inducible genes known to influence hematopoietic cell survival were quantified. High constitutive expression of the IFNgamma-inducible genes, IFN-stimulated gene factor 3 gamma su...
متن کاملEndogenous RD-114 virus genome expression in malignant tissues of domestic cats.
Endogenous xenotropic cat type C virus (RD-114)- and infectious feline leukemia virus (FeLV)-specific gene expressions were measured in spontaneous sarcomas carcinomas, and nonmalignant cat tissues by molecular hybridization for virus-specific RNA and competition radio-immunoassays for the major internal protein (p30) of these two viruses. The results indicate that RD-114 gene expression in sar...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1987
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.61.5.1524-1531.1987